isolation, purification, characterization and effect upon hepg2 cells of anemaran from rhizome anemarrhena

نویسندگان

qianqian jiang tianjin key laboratory for modern drug delivery and high-efficiency, school of pharmaceutical science and technology, tianjin university, tianjin 300072, china.

yunping zhao 1- tianjin key laboratory for modern drug delivery and high-efficiency, school of pharmaceutical science and technology, tianjin university, tianjin 300072, china. 2- school of chinese medicine, tianjin university of traditional chinese medicine, 300193, china.

wenyuan gao tianjin key laboratory for modern drug delivery and high-efficiency, school of pharmaceutical science and technology, tianjin university, tianjin 300072, china.

xia li tianjin key laboratory for modern drug delivery and high-efficiency, school of pharmaceutical science and technology, tianjin university, tianjin 300072, china.

چکیده

the rhizome of anemarrhena asphodeloides is used as food and traditional chinese medicine for its hypoglycemic effect. the aim of this study was to investigate the isolation, purification and hypoglycemic activity of anemaran as the active component. the influence factors (isolation duration, ratio of residuals to water and extracting times) during the isolation process were evaluated. the optimal conditions for na and aa were extraction temperature 90ºc and 100ºc, duration 1h and 1.5 h, extraction time 3 and 3, and the solid–liquor ratio 1:20 and 1:15, respectively. neutral and acid anemaran (na and aa) were isolated from the rhizome of anemarrhena asphodeloides. five fractions of na-1, na-2, na-3, aa-1 and aa-2 were obtained after crude neutral and acid anemaran purified through deae-52 cellulose anion-exchange column. the characterizations of anemaran and its different fractions were both analyzed by fourier transform infrared spectroscopy (ft-ir) and scanning electron micrographs (sem). structural properties of different fractions were examined by ft-ir. strong characteristic absorption peaks were observed at around 1744 cm−1and 1650 cm−1 caused by the c=o group of uronic acids, and the band between 1440 cm−1 and 1395 cm−1 associated with the stretching vibration of c–o of galacturonic acid. neither the crude neutral, nor the acid anemaran significantly inhibited the growth of hepg2 cells in-vitro, which indicated the low cytotoxicity of the anemaran. furthermore, both neutral and acid anemaran showed hypoglycemic effect. the hypoglycemic effect of neutral anemaran was much higher than that of acid anemaran.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isolation, Purification, Characterization and Effect upon HepG2 Cells of Anemaran from Rhizome Anemarrhena

The rhizome of Anemarrhena asphodeloides is used as food and traditional Chinese medicine for its hypoglycemic effect. The aim of this study was to investigate the isolation, purification and hypoglycemic activity of Anemaran as the active component. The influence factors (isolation duration, ratio of residuals to water and extracting times) during the isolation process were evaluated. The opti...

متن کامل

Isolation, Purification, Characterization and Effect upon HepG2 Cells of Anemaran from Rhizome Anemarrhena

The rhizome of Anemarrhena asphodeloides is used as food and traditional Chinese medicine for its hypoglycemic effect. The aim of this study was to investigate the isolation, purification and hypoglycemic activity of Anemaran as the active component. The influence factors (isolation duration, ratio of residuals to water and extracting times) during the isolation process were evaluated. The opti...

متن کامل

Isolation, Purification, Characterization and Effect upon HepG2 Cells of Anemaran from Rhizome Anemarrhena

The rhizome of Anemarrhena asphodeloides is used as food and traditional Chinese medicine for its hypoglycemic effect. The aim of this study was to investigate the isolation, purification and hypoglycemic activity of Anemaran as the active component. The influence factors (isolation duration, ratio of residuals to water and extracting times) during the isolation process were evaluated. The opti...

متن کامل

The apoptotic effect of sarsasapogenin from Anemarrhena asphodeloides on HepG2 human hepatoma cells.

Sarsasapogenin, a kind of mainly effective components of Anemarrhena asphodeloides Bunge (Liliaceae) has the effects of being anti-diabetes and improving memory. However, there are few reports focusing on its anti-tumor effects. In this study, the sarsasapogenin was extracted from rhizomes of A. asphodeloides Bunge and applied to inhibit HepG2 human hepatoma cells. MTT assay showed that sarsasa...

متن کامل

A Simplified Protocol for the Purification of Schwann Cells and Exosome Isolation from C57BL/6 Mice

Background: The purification of Schwann cells has proven to be a difficult process, with most methods requiring the use of special equipment. However, obtaining a sufficient number and high purity of Schwann cells is an integral aspect in their use for clinical application. Therefore, the aim of this study was to establish a simple and effective protocol for the isolation and purification of Sc...

متن کامل

Isolation, Purification and Characterization of a Thermophilic Alkaline Protease from Bacillus subtilis BP-36

The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of pharmaceutical research

جلد ۱۲، شماره ۴، صفحات ۷۷۷-۷۸۸

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023